![]() Its main working principle can be briefly summarised as follows: Cre mediates the removal of transcription termination DNA sequences on the LoxP side, whereupon predesigned reporter genes are expressed in cells expressing Cre ( Meinke et al., 2016). The Cre/LoxP recombinase system is widely used for in vivo tracking of stem or progenitor cell lines. Here, we describe the advances in cell lineage tracing technology, with a focus on Cre/LoxP recombinase systems ( Figure 1).Ģ.1 Genetic lineage tracing has entered the era of the recombinase system It has been used for detailed in vivo single-cell analysis because of its efficiency and spatiotemporal specificity ( Yao et al., 2020 Li et al., 2022a Geiller et al., 2022). Optogenetic engineering has been developed to some extent. Cell lineage tracing techniques have used classical fluorescent protein markers combined with microscopy to identify a few cell clones and to introduce heritable DNA barcodes into single cells to track larger numbers of more complex cell clones, the latter including Cre-mediated recombination and CRISPR-Cas9-mediated editing ( Meinke et al., 2016 Baron and van Oudenaarden, 2019). Lineage tracing is a powerful means of monitoring cells in various physiological and pathological processes, resulting in many valuable biological discoveries ( Kumar et al., 2014 He et al., 2017a Wu et al., 2021 Hou et al., 2022). 2 Tracking the targets all the way: lineage tracing ![]() Based on the latest literatures, stems cells referred in this review focus on adult tissue-specific stem cells. We do not provide a summary assessment of all relevant literature, and references throughout the text tend to be more illuminating examples. Therefore, this paper also combs the biological understanding of adult tissue-specific stem cells interactions. In addition, there are few systematic descriptions of the mechanisms of stem cell interactions. Specifically, these techniques or methods include lineage tracing, synthetic receptor systems, proximity labeling, and transcriptome analysis. In this paper, we first discuss the methods for studying adult tissue-specific stem cells interactions in two directions: bioinformatics analysis and visualization analysis. An enhanced understanding of the mechanisms of adult tissue-specific stem cells interaction is important for tissue regeneration and maintenance of homeostasis in organisms.Īdult tissue-specific stem cells interactions can be viewed as communication modalities that play a central role in regulating cell behavior and function ( Xin et al., 2016). This review aimed to describe the recent methodological advances of dual enzyme lineage tracing system, the synthetic receptor system, proximity labeling, single-cell RNA sequencing and spatial transcriptomics in the study of adult tissue-specific stem cells interactions. These technological innovations have prompted researchers to re-evaluate previous experimental results, providing increasingly compelling experimental results for understanding the mechanisms of cell-cell interactions. Recent advances in lineage tracing, synthetic receptor systems, proximity labeling, and transcriptomics have enabled easier and more accurate cell behavior visualization and qualitative and quantitative analysis of cell-cell interactions than ever before. How adult tissue-specific stem cells with indicated markers contact the adjacent lineage with indicated markers is of significance to be studied. Various in vivo markers of adult tissue-specific stem cells have been increasingly reported by lineage tracing in genetic mouse models, indicating that marked cells differentiation is crucial during homeostasis and regeneration. State Key Laboratory of Oral Diseases, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Department of Cariology and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, ChinaĪdult tissue-specific stem cells play a dominant role in tissue homeostasis and regeneration.
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